It also appears to slow the rates of depolarization and repolarization of muscle cell membranes. Spectrum offers highly pure reagents suitable for biochemical research and analysis. Identification product name guanidine hydrochloride cat no. Urea vs guanidine hydrochloride gdnhcl in equilibrium denaturation experiments, the concentration of urea at midpoint of denaturation is always higher than that of gdnhcl. If the patient feels unwell or is concerned, obtain medical advice. It is not indicated for treating myasthenia gravis. Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. Keep in a tightly closed container, stored in a cooled, dry, ventilated area. Choose from over 850 chemical products in chemical grades, sizes and concentrations to meet your needs. However, some stronger bases can deprotonate it, such as sodium hydroxide. Guanidine hydrochloride is the hydrochloride salt form of guanidine, a strong basic compound with parasympathomimetic activity. Find patient medical information for guanidine oral on webmd including its uses, side effects and safety, interactions, pictures, warnings and user ratings. Guanidine hydrochloride cas no 50011 material safety data sheet sds msds section 1. Guanidinium thiocyanate an overview sciencedirect topics.
Uses advised against not for food, drug, pesticide or biocidal product use details of the supplier of the safety data sheet emergency telephone number chemtreco, inside the. Guanidine hydrochloride is indicated for the reduction of the symptoms of muscle weakness and easy fatigability associated with eatonlambert syndrome. Safety data sheet guanidine hydrochloride date prepared. Identification of the substancemixture and of the companyundertaking 1. Bp178500 casno 50011 synonyms no information available recommended use laboratory chemicals.
Therefore, the benefits of therapy must be weighed against the potential hazards. Your new online msds binder is a place for you to store the material safety data sheets you need to deploy. Safety data sheet according to federal register vol. Each tablet contains 125 mg of guanidine hydrochloride with no color additive in the base. Safe use of guanidine hydrochloride in pregnancy has not been established. Guanidine hydrochloride material safety data sheet cas no 50. Guanidinium chloride is a weak acid with a pk a of. Guanidine hydrochloride, biotechgrade is considered a chaotrope and a strong denaturants utilized in physiochemical studies of protein folding. Add small amounts of water until solid is entirely dissolved 4. Being a so called chaotropic agent guanidine hcl is used as a powerful protein denaturant in the purification of proteins and nucleic acids dna and rna. It acts as an intermediate in the preparation of sulfadiazine, which is an important raw material for sulfamethyldiazine, sulfamethazine and folic acid. Symptoms of poisoning may even occur after several hours.
It may appear as a freeflowing crystalline powder, a freeflowing powder with solid material dispersed throughout, or a solid. Wear appropriate protective eyeglasses or chemical safety goggles as described by oshas eye and face protection regulations in 29 cfr 1910. It apparently acts by enhancing the release of acetylcholine following a nerve impulse. Proteins become randomly coiled at high concentrations of guanidinium chloride.
The most effective method for inactivating endogenous nucleases and deproteinizing rna is by rapid homogenization in the presence of guanidinium thiocyanate or guanidine hydrochloride macdonald et al. Guanidine hcl is used in the isolation of rna to dissociate the nucleoprotein into its nucleic acid and protein. Product name guanidine hydrochloride, for molecular biology 1. Chemically, guanidine aminomethanamidine hydrochloride is a crystalline powder freely soluble in water and alcohol. Guanidine thiocyanate sc202638 hazard alert code key. Measure concentration of solution by refractive index measurements pace cn, meth. Guanidine hydrochloride is used for purification of proteins and nucleic acids.
Practical products supplied by spectrum are indicative of a grade suitable for general industrial use or research purposes and typically are not suitable for human consumption or. I have eluted this glycoprotein from the protein gbound antibody using 6 m guanidine hydrochloride. Isolation of intact rna, free of protein and dna, is the critical first step in analyzing steadystate levels of transgene mrna. Othersguanidine hcl is the most popular protein denaturant. The quality of the product does not appear to be affected and solutions prepared from the freeflowing and lumpy guanidine hcl appear identical. Sigmaaldrich offers a number of guanidine hydrochloride products. Safety data sheet creation date 16nov2010 revision date 18jan2018 revision number 3 1. Articles of guanidine hydrochloride are included as well. No wasting valuable research time weighing and dissolving guanidine crystals free from uv absorbing materials in the range of 225300nm free from heavy metal contaminants fr. Any concentration below 8m guanidine can be quickly and ea. How do you run protein samples in 6m guhcl in sdspage. Extreme high moderate low section 1 chemical product and company identification product name guanidine thiocyanate statement of hazardous nature considered a hazardous substance according to osha 29 cfr 1910.
Guanidine hydrochloride, practical, also known as guanidinium chloride, is the hydrochloride salt of guanidine used in physiochemical studies of protein folding. As with most organic solids, fire is possible at elevated temperatures or by contact with an ignition source. Dec 02, 2019 fatal bonemarrow suppression, apparently dose related, can occur with guanidine. Carolina offers the highest quality kits for a handson approach within ap chemistry classrooms.
Other information revision 061917 modified effective date the information contained in this data sheet is based on the data available to puritan products at this time. Material may be irritating to the mucous membranes and upper respiratory tract. Thermo scientific pierce guanidine hcl can be easily dissolved and phadjusted to any concentration. Guanidine hydrochloride enhances the release of acetylcholine following a nerve impulse and potentiates acetylcholine actions on muscarinic and nicotinic receptors. Guanidine hydrochloride is used in rna isolation to dissociate nucleoproteins and inhibit rnase. Thermo scientific pierce 8m guanidine hcl solution is an accurately formulated, readytouse solution of guanidine hydrochloride, that can be easily diluted and phadjusted to any concentration below 8m. As with most organic solids, fire is possible at elevated temperatures or by contact with an ignition. The reason letting it such a weak acid is because of the complete delocalisation of the positive charge through 3 nitrogen atoms plus a little bit positive charge on carbon. Aminoformamidine hydrochloride, aminomethanamidine hydrochloride, guanidinium chloride cas number 50011. Because guanidine is excreted in milk, patients on this drug should discontinue breastfeeding. Personal protective equipment eyeface protection safety glasses with sideshields conforming to en166 use equipment for eye protection tested and approved under appropriate government standards such as niosh us or en 166eu. Carbon oxides, nitrogen oxides nox, hydrogen chloride gas.
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